Redox Metabolism Shared Resource Facility (RM SRF)
Director: D. Allan Butterfield, PhD
The mission of the Redox Metabolism Shared Resource Facility (RM SRF) is to provide expertise and services in redox metabolism, oxidative stress, mitochondrial function, proteomics, and metabolomics to basic and clinical investigators of the Markey Cancer Center (MCC) doing basic, pre-clinical, and clinical research.
Cancer cells demonstrate increased free radical biology and altered metabolism. Often these changes are manifested by elevated markers of oxidative stress, modified mitochondrial and cellular metabolism, and since proteins carry out metabolic processes, by differential protein expression and/or posttranslational modification. Among the latter are specific oxidative and epigenetic modifications.
To investigate these integrated aspects of redox metabolism, the RM SRF performs four major services:
- Analysis of markers of oxidative and nitrosative stress
- Seahorse FX-based analyses of mitochondrial function and glycolysis
- Proteommics or redox proteomics analyses of protein expression or oxidatively or covalently modified proteins
- Profiling and Stable Isotope Resolved Metabolomics (SIRM)
Request RM SRF Services
Contact Dr. Mihail (Mike) Mitov, RM SRF Operations Manager, via iLab for an initial consultation. At this consultation meeting, Dr. Mitov will direct you to the proper service component to help you get started. Click the link below, which will take you to the RM SRF iLab landing page, which contains more detailed instructions and a one-time account setup. Once
your account is set up, iLab will enable you to place RM SRF service requests,
provide the required approvals, and monitor the progress of your project.
How the RM SRF furthers Markey science
The role of free radicals and altered metabolism is increasingly apparent in various aspects of cancer biology and cancer therapy. Analysis of the roles of free radicals in cancer biology requires highly skilled ability to:
- Measure the damage induced by free radicals in various tissues, cells, and fluids associated with different cancers.
- Measure molecules responsible for free radical production, oxidant scavenging, and free radical damage.
- Use proteomics to identify proteins in tissues, cells, and fluids that have differential levels or have been oxidatively modified.
- Measure mitochondrial function, since mitochondria are a major source of free radicals in cells.
- Provide metabolic information about altered metabolites, enzymes, and pathways in cancer and following cancer treatments.
A fundamental cornerstone for the RM SRFs metabolomics component is stable-isotope labeling during the biological experiment, meaning experimental design must be integrated with analysis and informatics. Because of this, a simple fee-for-service model is unlikely to yield very useful
results for cancer researchers, and metabolomics services are structured for extensive advising and collaboration. Read more on the RM SRF Technologies page.
Importantly, and unique to this region of the United States, the RM SRF
is one of only a few cancer center-resident resources in the world with the
knowledge and reagents necessary to provide the redox and metabolism services
needed by MCC members. Personnel in the RM SRF are highly knowledgeable about
free radicals, oxidative stress, metabolism, sample handling and preparation,
and the advantages and limitations of each assay employed, including
bioenergetics, metabolomics, and proteomics. The RC-SIRM, housed in
the CESB, is one of the six metabolomics centers supported by the NIH Common
Fund. These unique investments provide
a distinct advantage to MCC researchers in sample use, analysis, and
interpretation of results.
If multiple PIs request simultaneous analyses, the RM SRF leadership determines priority based on the following criteria:
- Level 1: Samples from MCC members with NCI funding or cancer-related federally funded peer reviewed studies
- Level 2: Samples from MCC investigators preparing cancer-related, peer reviewed grant applications
- Level 3: Samples from MCC investigators with a cancer-related project funded by MCC pilot studies or non-peer reviewed source
- Level 4: Samples from MCC investigators without funding whose analysis would lead to preliminary data and the likelihood of a subsequent extramural proposal submission
- Level 5: Samples from non-MCC investigators.
Acknowledging the RM SRF
Investigators are required to acknowledge the Markey Cancer
Center Redox Metabolism Shared Resource Facility (MCC RM SRF)
in any publications that result from the use of Redox Metabolism services
or information received through the MCC RM SRF. For your convenience, you are
welcome to use the following statement. Please include the names of individuals from the RM SRF if they provided any intellectual input or additional effort.
The research was supported by the Redox Metabolism Shared
Resource Facility of the University of Kentucky Markey Cancer Center
Hours of operation
The RM SRF
can be contacted online any time, 24 hours a day, via iLab at https://ukmcc.ilabsolutions.com/service_center/show_external/3590/