Standard operating procedures

The Markey Biospecimen and Tissue Procurement Shared Resource Facility follows the standard operating procedures (SOPs) listed below. It is our policy to review and revise SOPs annually. Specific SOPs are created for the needs of a researcher’s protocol when necessary.

Tissue and liquid repository

Policies, safety, personnel

BSTP 24 New Employee Check-in Procedures (PDF, 67 KB) 

BSTP 67 Bloodborne Pathogen Occupational Exposure (Safety) (PDF, 26 KB) 

BSTP 70 General Chemical Safety, Electrical Hazards, and Back Safety (PDF, 23 KB) 

BSTP 76 Hazardous Liquid Nitrogen Event (PDF, 35 KB) 

BSTP 81 Medical Emergency and Work Place Injury (PDF, 24 KB) 

BSTP 87 Employee Check-Out Procedures (PDF, 23 KB) 

BSTP 88 Hand Washing And Hand Hygiene (PDF, 21 KB) 

BSTP 92 Password Guidelines (PDF, 24 KB) 

BSTP 109 Exposure Control Plan (PDF, 47 KB) 

BSTP 312 BSTP Spill Plan Process Document (PDF, 20 KB)  


BSTP 9 Standard Operating Procedure Template (SOP) (PDF, 22 KB) 

BSTP SRF 65 General Liquid Specimen Chain of Custody Form (PDF, 51 KB) 

BSTP SRF 66 General Surgical Path Chain of Custody Form (PDF, 38 KB)


BSTP 107 Cryomonitor and Cryomonitor Log (PDF, 85 KB) 

BSTP 203 Checking Liquid Nitrogen Levels, Ordering And Switching Out Liquid Nitrogen Source Tanks From the BSTP Cryostorage Units (PDF, 37 KB) 

BSTP 419 Autoclave Process Document (PDF, 21 KB) 

BSTP 420 Centrifuge Process Document (PDF, 23 KB)


BSTP SRF 63 General Protocol Collection Document IRB (PDF, 297 KB) 

BSTP SRF 195 Solid Specimen Macrodissection for Dispensation (PDF, 26 KB)

BSTP 317.1 Serial Number Sample Tracking System (PDF, 48 KB) 

BSTP 377 Dispensing Procedures For Sample Requests (PDF, 45 KB) 

BSTP 397 DNA Isolation Procedure (PDF, 97 KB) 

BSTP 408a GI SPORE Project 1 Phase 1 Detailed Protocol for University of Kentucky Participants (PDF, 72 KB)

BSTP 408b GI CPORE Project 1 Phase Detailed Protocol (Multisite) (PDF, 72 KB)

BSTP 415 DOD Lung Cancer Research Initiative (PDF, 64 KB)   

BSTP SRF 435 Application Review Process SOP (PDF, 29 KB)

BSTP SRF 439 Consenting in Markey Clinics for General Protocol IRB (PDF, 45 KB)     



Application Form Request for Biospecimens Histology IHC (PDF, 31 KB)

Equipment Verification of Training (PDF, 26 KB)

Skills Training Checklist (PDF, 55 KB)


Alcian Blue (PDF, 141 KB)

Hematoxylin and Eosin Staining (PDF, 47 KB)

Rapid Cold Method Preparation of Harris' Hematoxylin (PDF, 37 KB)

IHC with Envision Plus Kit (PDF, 69 KB) 

New Antibody Validation (PDF, 37 KB)

IHC with Vectastain Kit (PDF, 73 KB)

PAS for Glycoproteins (PDF, 49 KB)

Trichrome Massons (PDF, 49 KB)

Garvey (PDF, 43 KB)

Double Stain IHC (PDF, 67 KB)

IHC for IGF2BP1 (PDF, 38 KB) 

IHC for MRP2 (PDF, 34 KB)  

FLEX Antibodies (PDF, 287 KB) 

Digital imaging

Aperio Scanning Protocol (PDF, 62 KB)        

Page last updated: 10/16/2015 1:17:32 PM